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Immunologic Functions of Isolated Human Lymphocyte Subpopulations

III. Specific Allogeneic Lympholysis Mediated by Human T Cells Alone¹

From the Division of Tumor Immunology, Sidney Farber Cancer Center and the Department of Medicine, Harvard Medical School, Boston, Massachusetts

Abstract

The studies presented herein have evaluated both the specificity and cellular basis of cell-mediated lympholysis (CML) in man. An efficient and quantitative ⁵¹Cr release assay was utilized to study the role of highly purified human T and B cells in CML. After in vitro sensitization human T cells develop the capacity to kill specifically allogeneic cells to which they were sensitized. In contrast, B cells were neither triggered to proliferate nor activated to kill allogeneic targets. B cells were not activated to kill even when sensitized in the presence of potentially "helper" T cells, nor did they block T cells from killing during the effector phase. Cell-free supernatants taken from active in vitro sensitization cultures were not lympholytic and did not modulate T cell killing. Hence, these studies show that both the afferent and efferent phases of human CML are T cell functions.

Footnotes

¹ This work supported in part by Grants AI-12069 and CA-05167, and Contract 43964 from the National Institutes of Health, Bethesda, Maryland.