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Immune Response to Liposomal Model Membranes: Restricted IgM and IgG Anti-Dinitrophenyl Antibodies Produced in Guinea Pigs¹

From the Departments of Pharmacology, Pathology, and Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110

Abstract

Guinea pigs were immunized with liposomal model membranes actively sensitized with 2,4-dinitrophenyl-aminocaproylphosphatidyl-ethanolamine. The immune response was characterized by the formation of both IgM and IgG anti-DNP plaque-forming cells (PFC) which paralleled the appearance of anti-DNP antibodies in the serum. Plaque inhibition by DNP-lysine indicated that the PFC produced after liposomal immunization fell in a much narrower range of avidity groups than the PFC obtained after immunization with DNP-albumin and, in this regard, resembled MOPC 315. The restricted nature of the serum IgG anti-DNP antibodies was confirmed by isoelectric focusing and revealed the expression of a limited number of clones in all cases.

Footnotes

¹ The study was supported by Public Health Service Grants AI-09319, AI-11635, CA-16032 and a grant from the following companies: Brown & Williamson Tobacco Corporation: Larus and Brother Company, Inc.; Liggett & Myers, Inc.; Lorillard, A Division of Loews Theatres, Inc.; Philip Morris, Inc.; R. J. Reynolds Tobacco Company; United States Tobacco Company; and Tobacco Associates, Inc.