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Induction of Lymphocyte Cytotoxicity by Modification of the Effector or Target Cells with Periodate or with Neuraminidase and Galactose Oxidase

From the Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel

Abstract

Treatment of mouse spleen cells with periodate (NaIO₄) or with neuraminidase and galactose oxidase (NAGO) induces blastogenesis and renders the cells cytotoxic to mastocytoma (P815) target cells. Treatment of target cells (P815 cells and turkey erythrocytes) with NaIO₄ or with NAGO renders them susceptible to cytolysis by untreated mouse spleen cells. The cytotoxicity induced by NaIO₄ is reduced upon reacting the NaIO₄-treated, effector or target cells with borohydride or hydroxylamine. Thus the formation of free surface aldehydes on either the effector or target cell induced a cytotoxic effect. It is postulated that cross-linkage via a Schiff base between effector and target cell initiates the cytotoxic effect. Cytotoxicity induced by NaIO₄ or NAGO is immunologically nonspecific and is independent of major antigenic differences between effector and target cells. Phagocytic cells are not involved in NaIO₄-or NAGO-induced cytotoxicity toward P815 target cells.