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Rabbit Lymphocyte Populations Responding to Haptenic and Carrier Determinants for DNA Synthesis¹

From the Department of Medicine and Microbiology, The Johns Hopkins University School of Medicine at the Good Samaritan Hospital, Baltimore, Maryland 21239

Abstract

Mesenteric lymph node cells from rabbits primed with dinitrophenyl derivative of either Ascaris extract (DNP-Asc) or ragweed fraction D (DNP-Rag) were stimulated by the priming antigen, free homologous carrier, or with the hapten conjugated with rabbit serum albumin (DNP-RSA), and increase in DNA synthesis was observed by ³H-thymidine incorporation. The results showed that both free carrier and DNP-RSA stimulated DNA synthesis. The response of the primed lymph node cells to DNP-homologous conjugate was slightly higher than that to free carrier, but the optimal concentration of both antigens for maximal thymidine incorporation was 10 to 100 µg/ml. This concentration was about 100 times higher than the optimal concentration of the same antigens for maximal antibody response in vitro. The DNA synthetic response to DNP-RSA was much less than that obtained by free carrier, and the optimal concentration of DNP-RSA for the response was comparable to the concentration of DNP-homologous carrier conjugate to induce maximal anti-DNP antibody response. The relative importance of immunoglobulin bearing (B) cells and T cells in the DNA synthetic responses was assessed by fractionating lymph node cells with antigen-coated or antiimmunoglobulin coated cellular immunosorbent. The results indicated that hapten-specific, immunoglobulin-bearing cells (B cells) are responsible for the DNA-synthetic response to DNP-RSA, whereas B cells play a minimal role in the response to free carrier.

Footnotes

¹ This work was supported by research Grant AI-11202 from the United States Public Health Service and a grant from The John A. Hartford Foundation. This is publication No. 147 from the O'Neill Laboratories at the Good Samaritan Hospital.

² Awardee of International fellowship IF-05TW01917 from the U.S. Public Health Service. Present address: Department of Immunology Hospital St. Pierre, Rue Haute, Bruxelles, Belgium 1006.