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T Mitogens Trigger LPS Responsiveness in Mouse Thymus Cells¹

From the Tumor Biology Unit Department of Pathology, University of Florida College of Medicine, ¹ Gainesville, Florida 32610

Abstract

Mouse thymus cells are essentially unresponsive to lipopolysaccharide (LPS) stimulation. However, when cultured with minimally mitogenic levels of concanavalin A or submitogenic ratios of mitomycin-treated allogeneic spleen cells, in combination with LPS, they demonstrate levels of DNA synthesis de novo or greater than those induced by the T cell mitogen alone. Dose-response kinetics were characteristic of LPS. The subpopulation containing the LPS responsive cells was of low net buoyant density. Neither phytohemagglutinin nor pokeweed mitogen acted synergistically with LPS in this model to trigger thymus cells. The data suggest that LPS triggering may involve interaction with a T cell subpopulation.

Footnotes

¹ This is publication No. 88 from the Tumor Biology Unit.

² Doctor Nakao's present address is: Third Division, Department of Internal Medicine, School of Medicine, Kobe University, Ikuta-ku, Kobe, Japan.

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