HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by McKenzie, I. F. C. Articles by Snell, G. D. Search for Related Content PubMed Articles by McKenzie, I. F. C. Articles by Snell, G. D.

Ly-4.2: A Cell Membrane Alloantigen of Murine B Lymphocytes

I. Population Studies¹

From the Transplantation Unit and the General Surgical Services, Massachusetts General Hospital, and the Department of Surgery, Harvard Medical School, Boston, Massachusetts 02114, and the Jackson Laboratory, Bar Harbor, Maine 04609²

Abstract

The Ly-4 locus defines an alloantigenic specificity present on the surface of lymphocytes of some mouse strains and detected in lymphocytotoxicity tests by an antiserum (BALB/c x SWR)F₁ anti-B10.D2. In this paper are presented results which indicate that Ly-4.2 is predominantly represented on B cells and not T cells. In cytotoxicity tests with rabbit complement, the distribution of Ly-4.2 is restricted: thymus 5%, TDL 12%, spleen 60%, lymph node 30%, bone marrow 36%, Peyer's patches 55%, peritoneal exudate cells 35%, and peripheral lymphocytes 35%. This distribution is the reciprocal of that obtained with anti-Thy-1 antiserum. Further, when both reagents were used together, almost all cells in these tissues were lysed, excepting those with bone marrow. When anti-Ly-4.2 and anti-Thy-1.2 were used sequentially, it was apparent that these specificities were present on different cell populations. As Thy-1 is a marker for T cells, Ly-4 is therefore, presumably, a marker for B cells. This conclusion was confirmed by testing mice depleted of T cells ("nude"; thymectomy + anti-lymphocyte serum treatment; thymectomy, irradiation + reconstitution with bone marrow) where the majority of lymphocytes were Ly-4.2⁺, Thy-1.2^-; and by testing tumors, where, for the most part, this reciprocal relationship held true. Appropriate studies indicate that the Ly-4.2 antibody does not detect a surface immunoglobulin allotype. Anti-Ly-4.2 antiserum may provide a useful alloantigenic marker distinct from immunoglobulin, mouse-specific bone marrow-derived lymphocyte antigen, and "" for detecting some or all, B cells.

Footnotes

¹ This investigation was supported in part by National Institutes of Health Research Grants AI-10904 from the Institute of Allergy and Infectious Diseases and by CA-01329 from the National Cancer Institute.

² The Jackson Laboratory is fully accredited by The American Association for Accreditation of Laboratory Care.

This article has been cited by other articles:

				R. Hyman and I. Trowbridge Analysis of Lymphocyte Surface Antigen Expression by the Use of Variant Cell Lines Cold Spring Harb Symp Quant Biol, January 1, 1977; 41(0): 407 - 415. [Abstract] [PDF]