HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Till, G. Articles by Ward, P. A. Search for Related Content PubMed Articles by Till, G. Articles by Ward, P. A.

Two Distinct Chemotactic Factor Inactivators in Human Serum¹

From the Department of Pathology, University of Connecticut Health Center, School of Medicine, Farmington, Connecticut

Abstract

The chemotactic factor inactivator (CFI) has been isolated from whole human serum by a combination of techniques including salt precipitation, anionic exchange, and gel filtration chromatography. Two inactivators have been obtained, a -globulin with a sedimentation velocity of approximately 7S and an -globulin with a sedimentation velocity of approximately 4S. The former has a specificity for inactivation of the chemotactic activity associated with the C3 fragments, whereas the C5 chemotactic fragment is specifically inactivated by the -globulin CFI. CFI in crude fractions of human serum is heat labile, time and temperature dependent for its activity, and pH dependent, expressing optimal activity at a pH range of 7.2 to 7.4.

Footnotes

¹ This work was supported in part by National Institutes of Health Grants AI 09651 and AI 11526

² G. T. is supported by Research Fellowship Grant Ti 63/4 from the Deutsche Forschungsgemeinschaft.