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Suppression of Reaginic Antibody Formation

II. The Use of Adoptive Transfer System for the Study of Immunological Unresponsiveness¹

From the MRC Group for Allergy, Department of Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada

Abstract

By the use of the adoptive transfer system in syngeneic mice it was demonstrated that: i) spleen cells of animals tolerized by the i.v. injection of DNP₈-MG 1 month before adoptive transfer into x-irradiated recipients remained unresponsive when further challenged with the sensitizing antigen (DNP_2.3-OA administered i.p. with Al (OH)₃); by contrast, a significant response (typical of a secondary response) was obtained in recipients of cells from mice which had received only the sensitized antigen; ii) the anti-hapten IgE secondary response of primed cells was not affected by the presence of cells of tolerized mice when these two types of cells were administered and challenged together in x-irradiated recipients; iii) the transfer of spleen cells or serum of mice, tolerized 20 days before harvesting, into intact syngeneic recipients did not interfere with the normal development of the anti-DNP IgE response of the latter; iv) the anti-hapten responses of tolerized animals receiving spleen cells from normal or primed mice together with the sensitizing antigen resulted, respectively, in a primary or secondary response; (v) the unresponsive state of tolerized cells, achieved by immunosuppression of the original donors with respect to the haptenic determinant, was maintained even after two serial exposures of the cells to the immunizing antigen in two consecutive adoptive cell transfers into x-irradiated recipients. These findings provide a strong basis for the interpretation that the hapten-specific tolerance induced in mice by treatment with DNP₈-MG involved the elimination or inactivation of hapten-specific IgE-producing cells or the blockade of the receptors of these cells.

Footnotes

¹ This investigation was supported by grants from The Medical Research Council of Canada. The results of this study were presented at the 10th Symposium of the Collegium Internationale Allergologicum, Copenhagen, Denmark, August, 1974.

² Holder of a Scholarship from the Medical Research Council of Canada.