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The Journal of Immunology, 1975, 114: 776-778.
Copyright © 1975 by The American Association of Immunologists, Inc.
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Rat Cell Surface Antigens

I. Isolation and Partial Characterization of an Ag-B Antigen1

Gerald N. Callahan and Charles W. Dewitt

From the Department of Pathology, College of Medicine, University of Utah, Salt Lake City, Utah 84132

Abstract

Rat major histocompatibility or Ag-B antigen was isolated from soluble extracts of lymphoid cells by affinity chromatography. The purified antigen was shown in SDS gel electrophoresis to have a molecular weight of 30,000 to 35,000 daltons. In addition, amino acid composition was found to resemble, generally, that of HL-A and H-2 antigens. It thus appears that the demonstrated homology between H-2 and HL-A extends also to Ag-B.

Footnotes

1 This work was supported by Grants AI 11956 and AI 08934 from the National Institute of Allergy and Infectious Diseases.






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