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Anti-Receptor Antibody

I. Isolation and Characterization of the Immunoglobulin Receptor for Phosphorylcholine¹

From University of Chicago, La Rabida Institute and Departments of Pathology and Biochemistry, E. 65th Street at Lake Michigan, Chicago, Illinois 60649, and the Department of Microbiology, University of Texas Southwestern Medical School, Dallas, Texas 75230

Abstract

We have demonstrated a phosphorylcholine-binding protein in lysates from radioiodinated splenocytes of immunized mice. Immunoprecipitation with anti-receptor antibody of lysates from splenocytes obtained from animals undergoing a primary response or from mice immunized 4 to 6 months earlier with antigen demonstrated that this protein contained H and L chains.

Therefore, we have isolated an antigen-specific receptor from the surface of spleen cells from both immunized and "memory" animals. This receptor Ig comprises approximately 10% of total cell surface Ig.

Footnotes

¹ This work was supported by United States Public Health Service Grants AI-11080, AI-10967, and AI-11851.

² MSTP Trainee supported by United States Public Health Service Grant 2TO5GM0 1939-06.

³ Recipient United States Public Health Service Career Development Award AI-70559.