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The Journal of Immunology, 1975, 114: 99-101.
Copyright © 1975 by The American Association of Immunologists, Inc.
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Equine Anti-Hapten Antibody

IX. IgM anti-Lactose Antibodies1

Ming-Ming Chua, Don O. Morgan2 and Fred Karush3

Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19174 and the Department of Veterinary Sciences, College of Agriculture, University of Kentucky, Lexington, Kentucky 40506

Abstract

The immune response to a bacterial vaccine of Streptococcus faecalis (strain N) was characterized in all of the seven horses studied by the sustained production of about 90% IgM anti-lactose antibody over a period of 44 weeks with maximum values of the total antibody ranging from 4 mg/ml of serum to 12 mg/ml of serum. With respect to the binding of a lactose-containing ligand the association constants of the antibodies purified from sera obtained between 5 and 44 weeks fell in the range of 1 x 105 M-1 to 2 x 105 M-1. Not only was there no significant indication of maturation of affinity in this period but there was a selective limitation of affinity compared to that of 7S antibodies. It was inferred that the synthesis of IgM antibody involves the selective utilization of VH and/or VL genes.

Footnotes

1 This work was supported by Public Health Service research Grant AI-09492 from the National Institute of Allergy and Infectious Diseases and by a research grant from the Grayson Foundation, Inc.

2 Present address: Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, University of Illinois, Urbana, Illinois 61801.

3 Recipient of a Public Health Service research career award (5-K6-AI-14,012) from the National Institute of Allergy and Infectious Diseases.






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