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The Journal of Immunology, 1975, 114: 5-9.
Copyright © 1975 by The American Association of Immunologists, Inc.
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A Radioimmunoassay for Human Antigen-Antibody Complexes in Clinical Material1,2,

John S. Cowdery, Jr., Perry E. Treadwell and Robert B. Fritz

Department of Microbiology, Emory University, Atlanta, Georgia 30322

Abstract

Soluble antigen-antibody complexes have been detected by a radioimmunoassay with rheumatoid factor as the primary antibody to bind 125I immune complexes or heat aggregated IgG. Bound complexes are precipitated by rabbit anti-human IgM. The binding of the rheumatoid factor to the iodinated altered IgG can be inhibited by preincubation of the rheumatoid factor with human antigen-antibody complexes. The degree to which immune complexes inhibit the binding of rheumatoid factor to heat aggregated IgG has been shown to be dependent upon the antigen-antibody ratio of the complexes, with complexes formed at or near the point of equivalence exhibiting maximum inhibitory activity. Sera from individuals suffering from certain connective tissue disorders were found to inhibit the binding of rheumatoid factor to 125I aggregated IgG; sera from normal individuals did not inhibit this reaction when used at comparable dilutions. This procedure can be used as a relatively simple, reproducible means of detecting immune complexes in clinical specimens. The assay is sufficiently sensitive to detect 125 ng of soluble immune complexes.

Footnotes

1 Publication 1223, Division of Basic Health Sciences, Emory University.

2 This work was supported in part by U. S. Public Health Service Research Grant CA 12078 from the National Cancer Institute.






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