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Interaction of IgE with Rat Basophilic Leukemia Cells

III. Release of Intact Receptors on Cell-Free Particles

Arthritis and Rheumatism Branch, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, Bethesda, Maryland 20014

Abstract

Rat basophilic leukemia cells which bind rat IgE-release particulate material as they lose viability. This material appears to contain the surface receptors for IgE in their native form. Up to 80% of the receptor activity has been recovered in a fraction which contains only 4 to 5% of the total cellular protein.

Footnotes

¹ Present Address: Department of Medicine, University of California at San Diego, San Diego, California 92110.

² Present Address: Department of Medicine, Washington University School of Medicine, St. Louis, Mo. 63110.

³ To whom correspondence should be addressed.