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The Journal of Immunology, 1975, 114: 10-16.
Copyright © 1975 by The American Association of Immunologists, Inc.
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The Molecular Dimensions of Mitogenic Factor from Guinea Pig Lymph Node Cells1

Celia L. Gately2, Maurice K. Gately2 and Manfred M. Mayer

Department of Microbiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Abstract

The molecular dimensions of a mitogenic factor from guinea pig lymph node cells were investigated by means of gel filtration, sucrose density gradient ultracentrifugation, and CsCl isopycnic ultracentrifugation. The mitogenic factor was produced by stimulation of immune lymph node cells from inbred guinea pigs, strain 2 or 13, with the specific antigen, ovalbumin; and mitogenic activity was assayed by measuring incorporation of tritiated thymidine by target lymph node cells from syngeneic animals not immune to ovalbumin. The D20,w of the mitogenic factor was estimated to be (9.9 ± 0.2) x 10-7 cm2/sec by gel filtration on Sephadex G-100, and the apparent S20,w was estimated to be 2.4 ± 0.2 S by sucrose density gradient ultracentrifugation. The buoyant density was determined to be 1.324 ± 0.006 g/ml by CsCl isopycnic ultracentrifugation, and from this value the partial specific volume of mitogenic factor was estimated to be 0.71. From these data the molecular weight of the mitogenic factor was calculated to be 20,000, and the frictional ratio was calculated to be 1.2.

Footnotes

1 This work was supported in part by a Contract with the Office of Naval Research N00014-67-A-0163-0014 and United States Public Health Service Training Grant M 5T01 AI00282-10.

2 Recipient of Pre-Doctoral Fellowship on United States Public Health Service Training Grant M 5 T01 AI00282-10.






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