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A Pathologic IgM Occurring in Serum as Pentamer, Dimer, and Monomer¹

From the Departments of Biochemistry and Medicine, University of Toronto, Toronto, Ontario, Canada M5S 1A8

Abstract

IgM was recovered from the serum of a patient (Den) with Waldenstrom's macroglobulinemia by preparative electrophoresis and gel filtration. In the latter step three species were resolved, with sedimentation coefficients of 7.7S, 11.5S, and 19.0S. Molecular weight determinations showed that the three species were monomeric, dimeric, and pentameric IgM, respectively. The 19S and 11S species were not dissociated in 6M urea. Serologic, immunologic, and biochemical studies indicated that the constituent light and heavy chains of each species were the same. Light chains prepared from 19S IgM (Den) contained a second component identified as J chain. Light chains from 11S and 7S IgM (Den) did not contain this component.

Footnotes

¹ This work was supported by Grant MT 694 from the Medical Research Council of Canada.