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The Antibody Response to Myelin Basic Protein in Lewis and Fischer Rats as Followed by a Sodium Sulfate Method of Radioimmunoassay¹

From the Laboratory of Neuroimmunology, Departments of Microbiology-Immunology and Surgery, Duke University Medical Center, Durham, N. C. 27710

Abstract

It was found that the sodium sulfate method of radioimmunoassay of ¹²⁵I-labeled myelin basic protein could be used in the measurement and linear regression analysis of myelin-basic protein-binding activity in Lewis and Fischer rats, and could be adapted to the day-to-day single point measurements of antibody in microliter quantities of serum without untoward loss of statistical precision.

Footnotes

¹ This work was supported by Grant 833-A1 from the National Multiple Sclerosis Society and by Public Health Service Research Grant NS-10237 from the National Institute of Neurological Diseases and Stroke.