HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Thornthwaite, J. T. Articles by Leif, R. C. Search for Related Content PubMed Articles by Thornthwaite, J. T. Articles by Leif, R. C.

The Plaque Cytogram Assay

I. Light and Scanning Electron Microscopy of Immunocompetent Cells¹

From the Papanicolaou Cancer Research Institute, 1155 N.W. 14th Street, Miami, Florida 33123

Abstract

A method, the plaque cytogram assay, for preparing fixed, stained dispersions of plaque-forming cells (PFC) and rosette-forming cells (RFC) is described. Both light and scanning electron microscopy (SEM) studies demonstrate that these cells are pleomorphic. Eight types of PFC and seven types of RFC can be simultaneously identified by light microscopy in which small and medium lymphocytes are the predominate cell types of the two immunocompetent cell classes. SEM shows fairly smooth cell surfaces on the PFC, while the RFC have a rough surface. Enzymatic staining of PFC and RFC for lipase demonstrates that none of the PFC are stained positive for lipase, while approximately 27% of the RFC are lipase positive. Finally, several distinct types of plaques and rosettes are shown in the plaque cytogram assay.

Footnotes

¹ This work was supported in part by National Institutes of Health Grants CA 13441 and GM 18671 and Damon Runyon-Walter Winchell Grant DRG-1232.

² In partial fulfillment for the Ph.D. degree in the Department of Chemistry, Florida State University, Tallahassee, Florida.

³ Departments of Microbiology and Biomedical Engineering, University of Miami, Miami, Florida.