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Differentiation of Lymphoid Cells: The Use of Specific Antisera to Characterize the Cells Required for the Induction of Immunoglobulin M Production in Vitro

From the National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, Bethesda, Maryland 20014

Abstract

Anti-immunoglobulin as well as anti-thymocyte sera prevented the induction of immunoglobulin M production by rabbit popliteal lymph node cells that ordinarily occurs when such cells are cultured in vitro, in the absence of added antigen. Antibodies specific for the light chains and µ-chains of rabbit immunoglobulins were inhibitory whereas sera specific for - and -chains were without effect. Univalent anti-µ-chain antibody and univalent anti-thymocyte antibody preparations were also inhibitory. All inhibitory antisera were without effect when added to cultures after the induction process was completed. Thymidine incorporation studies showed a lack of correspondence between the inhibitory activity and mitogenicity of anti-immunoglobulins.

Footnotes

¹ Present address: Electronucleonics Laboratory, Inc., 4905 Delray Avenue, Bethesda, Maryland 20034.