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The Journal of Immunology, 1974, 113: 1883-1890.
Copyright © 1974 by The American Association of Immunologists, Inc.
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Receptors for Antigen on Lymphoid Cells

I. Immunoadsorption of Plaque-Forming Cells to Poly-L-Lysine-Fixed Antigen Monolayers1

Elizabeth Greeley, Gideon Berke2 and David W. Scott

From the Division of Immunology, Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710

Abstract

A method was developed for examining the binding specificities of plaque-forming cells (PFC) which were immune to either goat red blood cell surface antigens or to the trinitrophenyl (TNP) haptenic group. Immune rat lymph node cells were plated on poly-l-lysine fixed red blood cell (RBC) monolayers which functioned dually as immunoadsorbents and as plaque indicators of both adherent and nonadherent PFC populations. Anti-TNP PFC demonstrated marked specific adherence to TNP-GRBC. The adherence was optimal following a 30-min adsorption phase and was essentially independent of the adsorption temperature in the 4° to 37°C range. Specific adherence of anti-GRBC PFC to GRBC monolayers at 4°C was not detectable, but did occur at adsorption temperatures of 25° or 37°C. Temperature differences in specific adsorption in the two systems were not due simply to differences in the density of antigenic determinants on the adsorbing monolayers. Specific adherence of anti-GRBC PFC at 4°C was able to occur in the presence of DEAE-dextran, indicating the importance of surface charge effects. This immunoadsorbent-indicator system provides a means of studying the recognition events occurring at the PFC surface.

Footnotes

1 Supported by United States Public Health Service Grants AI-00285, AI-10716, and AI-08897.

2 Present address: Department of Cell Biology, The Weizmann Institute of Science, Rehovot, Israel.






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