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Anti-Idiotypic Antibody in Rat Transplantation Immunity¹

I. Production of anti-Idiotypic Antibody in Animals Repeatedly Immunized with Alloantigens

From the Departments of Pathology and Surgery and the Franklin McLean Memorial Research Institute, University of Chicago, Chicago, Illinois 60637

Abstract

Lewis x Brown Norway (LBN) F₁ antiserum to an idiotype of Lewis anti-BN alloantibody (referred to as the Id-1 idiotype) was used to test a variety of Lewis alloimmune sera for the presence of Id-1. Several such Lewis sera raised by BN antigen injection at monthly intervals possessed the Id-1 idiotype. Hyperimmune Lewis anti-BN alloantiserum raised by six weekly injections of BN antigen, however, did not possess the Id-1 idiotype, but appeared to possess anti-idiotypic antibody to Id-1 instead. The anti-BN hemagglutinin titer, anti-idiotypic antibody titer to Id-1, and levels of the Id-1 idiotype were then measured in individual weekly serum samples from Lewis rats repeatedly immunized with BN antigen. The anti-BN hemagglutinin titers followed a cyclical pattern, with the Id-1 idiotype predominating only in the first cycle of the anti-BN hemagglutinin response. Anti-idiotypic antibody was demonstrable in five of eight repeatedly immunized rats, and in each of these five animals the appearance of anti-idiotypic antibody to Id-1 followed the clearance of Id-1 from the serum. Anti-idiotypic antibody may play a role in the regulation or control of an established immune response.

Footnotes

¹ This work was supported by United States Public Health Service Research Grants AI-4197, AI-9268, and AI-08074, and Training Grant GM-00093.

² Operated by the University of Chicago for the United States Atomic Energy Commission.

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