HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Kateley, J. R. Articles by Friedman, H. Search for Related Content PubMed Articles by Kateley, J. R. Articles by Friedman, H.

Detection and Enumeration of Immunocytes Producing Different Classes of Vibriolysins after Primary and Secondary Immunization with Cholera Vaccine¹

From the Department of Microbiology, Albert Einstein Medical Center, Philadelphia, Pennsylvania 19141

Abstract

The vibriolytic plaque-forming cell (PFC) assay was used to study the kinetics of the primary and secondary immune responses of mice and rabbits immunized with heat-killed cholera vibrios. Immunocytes releasing IgG antibody could be detected as readily as those immunocytes secreting IgM antibody in spleens of BALB/c mice and New Zealand White rabbits after a single injection of vaccine. Peak numbers of indirect (IgG) PFC were detected 3 to 4 days after the peak direct (IgM) PFC response (12 to 14 days). In contrast, only direct vibriolytic PFC were detected in spleens of NIH Albino mice during the primary response to cholera antigens. After a second injection of vaccine, IgM, IgG, and IgA PFC were detected in both mouse strains with peak responses for each immunocyte class occurring within the first week after booster injection. Heat-killed vibrios or a lipopolysaccharide (LPS) extract, but not cholera exotoxin or E. coli LPS, inhibited the vibriolytic response. Furthermore, viable cholera vibrios were a more sensitive indicator cell in the immunoassay than sheep erythrocytes sensitized with a cellular extract from heated vibrios.

Footnotes

¹ This work was supported in part by research grants from the United States National Science Foundation and the National Institute of Allergy and Infectious Diseases.

² Present Address: Marmoset Research Center, Oak Ridge Associated Universities, P.O. Box 117, Oak Ridge, Tennessee 37830.