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Antibodies to Proteins Dissolved in Sodium Dodecyl Sulfate¹

From the Division of Biology, California Institute of Technology, Pasadena, California 91109

Abstract

Rabbits were injected with Drosophila embryo total nonhistone chromosomal (NHC) proteins or with bovine serum albumin, both in 0.1% sodium dodecyl sulfate (SDS). Antibodies were produced which reacted specifically with the protein injected as antigen; the antibodies were not directed against a random denatured protein-SDS complex as shown by failure to cross-react with other proteins in SDS. Antibodies to nonhistone chromosomal proteins were also produced by injecting rabbits with native chromatin. These antibodies reacted with isolated NHC proteins in 0.1% SDS. The presence of low concentrations of SDS did not seem to affect either production of antibodies or their general specificity. The antibodies produced in response to immunization with total NHC proteins or chromatin were directed against at least several proteins as determined by a procedure involving indirect immunofluorescent staining of antibody-antigen complexes in SDS polyacrylamide gels.

Footnotes

¹ This work was supported in part by The Jane Coffin Childs Memorial Fund for Medical Research and in part by National Science Foundation Grant GB 34160.

² Recipient of a National Science Foundation predoctoral fellowship.

³ Fellow of the Jane Coffin Childs Memorial Fund for Medical Research. Present address: Harvard University, The Biological Laboratories, 16 Divinity Avenue, Cambridge, Massachusetts 02138.

⁴ Recipient of National Institutes of Health Career Development Award A1-20388.