HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Preud'Homme, J.-L. Articles by Flandrin, G. Search for Related Content PubMed Articles by Preud'Homme, J.-L. Articles by Flandrin, G.

Identification by Peroxidase Staining of Monocytes in Surface Immunofluorescence Tests¹

Laboratoire d'Immunochimie (U 108 INSERM) and Laboratoire de Cytologie, Institut de Recherches sur les Maladies du Sang, Hôpital Saint-Louis, 2, place du Dr. Fournier, 75475 Paris Cedex 10

Abstract

Surface immunoglobulins (S.Ig)² on human lymphocytes have been widely studied in the last few years, mainly with surface immunofluorescence (IF) tests. These studies have led to somewhat contradictory results (reviewed in 1) and most discrepancies are likely explained by methodological reasons since S.Ig detection is exposed to a number of pitfalls (2). Monocytes represent an important source of potential errors. They bind serum IgG by their Fc receptor and therefore stain with conjugated antisera to -, - and -immunoglobulin chains. In addition some monocytes are able to bind rabbit IgG used as reagents and show positivity in IF tests whatever the specificity of the conjugate is. Two approaches may be used in order to avoid erroneous interpretation due to monocytes, either the use of cell suspensions free of monocytes or the identification of monocytic cells.

Footnotes

¹ This work was supported by I.N.S.E.R.M. (U 108) and D.G.R.S.T. (Grant 71.7.3065).

² Abbreviations used in this paper: S.Ig, surface immunoglobulin; IF, immunofluorescence; POX, peroxidase.

This article has been cited by other articles:

				J. Bekesi, J. Holland, H. Anderson, A. Fischbein, W Rom, M. Wolff, and I. Selikoff Lymphocyte function of Michigan dairy farmers exposed to polybrominated biphenyls Science, March 17, 1978; 199(4334): 1207 - 1209. [Abstract] [PDF]