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Antibody-Dependent Cell-Mediated Cytotoxicity

II. Early Interactions Between Effector and Target Cells¹

La Rabida University of Chicago Institute and the Department of Pathology, University of Chicago, Chicago, Illinois 60637

Abstract

The early interactions between effector and target cells in antibody-dependent cell-mediated cytotoxicity were analyzed in a system with hapten-coated target cells and anti-hapten antibodies. Direct contact between effector and target cells through the antibody molecule is required for lysis of target cells. Establishment of contact can be prevented by free hapten, but once contact has occurred, free hapten is ineffective in preventing lysis of target cells. After effector cell-target cell interactions, target cell damage is first manifested by increased osmotic fragility and later by a progressive, temperature dependent, increased permeability for intracellular components. Effector cells and target cells remain in contact during the lytic process, which is completed in several hours.

Footnotes

¹ This work was supported by the following United States Public Health Service Grants: AI-11080, AI-09268, AI-10242, and AI-10457, and American Chemical Society Grant IC 21.

² Present address: Department of Clinical Chemistry and Laboratory Medicine, M.D. Anderson Hospital, Houston, Texas 77025.