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The Journal of Immunology, 1974, 113: 1501-1509.
Copyright © 1974 by The American Association of Immunologists, Inc.
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Resistance of Sheep Erythrocytes to Immune Lysis by Treatment of the Cells with a Human Erythrocyte Extract: Studies on the Site of Inhibition1,2,

E. M. Hoffmann, W. C. Cheng, E. J. Tomeu and C. M. Renk

Department of Microbiology, College of Arts and Sciences, and the Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida 32611

Abstract

Sheep erythrocytes (E) were treated with an anti-complementary extract derived from human erythrocyte stromata (I-H) and then converted to the intermediate state
Figure 1
. It was found that the treated
Figure 2
decayed at about the same rate as control
Figure 3
which had been generated from untreated sheep E. However, the extent of lysis of the treated intermediate was less than that of the control. Subsequent studies indicated that the same number of SAC
Figure 4
was generated on treated E as was generated on control cells. Furthermore, the resulting EAC
Figure 5
consumed essentially the same amount of C2 in both cases, but
Figure 6
which had been formed from extract-treated sheep E consumed less C3 than did control
Figure 7
(cells not treated with I-H). It was also found that I-H would inhibit lysis of
Figure 8
but not
Figure 9
when the intermediates were formed and then treated with the extract.

Preliminary purification was attempted with a combination of DEAE chromatography and gel filtration with Sepharose 4B. However, yields of active material were quite low. It was estimated that the factor, as isolated, had a very large molecular weight.

Footnotes

1 This work was supported in part by United States Public Health Service Grant AM14244 and the Institute of Food and Agricultural Sciences.

2 Authorized for Publication as Paper 5373 in the Journal Series of the Florida Agricultural Experiment Station.






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