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The Genetic Control of Antibody Binding Constants and Specificities in Inbred Rats^1,2,

Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261

Abstract

The binding constants of antibodies to poly(Glu⁵²Lys³³Tyr¹⁵) from several strains of high and low responding rats and their various hybrids were measured by equilibrium dialysis. High responders produced antibodies with higher binding constants than low responders. Breeding studies showed that the ability to produce high affinity antibodies is under genetic control and is linked to the major histocompatibility locus. Aggregation of the antigen increased the quantity of antibody and its binding constant in the low responders and decreased both in the high responders. Precipitin reactions using various related synthetic polypeptides indicated that the antibodies produced by the high responders were more cross-reactive than those produced by the low responders. Breeding studies showed that this ability is also inherited in a dominant manner and is linked to the major histocompatibility locus.

Footnotes

¹ This paper is number seven in the series "Genetic and cellular factors in the immune response." The work was supported by Grants AI 10611 and GM 00135 from the National Institutes of Health.

² This work was done in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the University of Pittsburgh School of Medicine by Sandra K. Ruscetti.