The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 1974, 113: 1438-1445.
Copyright © 1974 by The American Association of Immunologists, Inc.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Davie, J. M.
Right arrow Articles by Paul, W. E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Davie, J. M.
Right arrow Articles by Paul, W. E.

Role of T Lymphocytes in the Humoral Immune Response

I. Proliferation of B Lymphocytes in Thymus-Deprived Mice1

Joseph M. Davie and William E. Paul

Departments of Pathology and Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110, and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Abstract

The humoral response to dinitrophenyl-keyhole limpet hemocyanin (DNP-KLH) was measured in thymectomized, irradiated, bone marrow-reconstituted, and rabbit antimouse thymocyte antiserum-(ALS)treated (Tx-BM) mice. The total anti-DNP antibody response, as measured by plaque-forming cells, was not severely decreased, but was changed in character from the normal predominantly IgG secondary response to a persistently IgM response. The IgG secondary response was partially restored to Tx-BM mice by injecting syngeneic thymocytes i.v. as late as 1 week before or activated thymus-derived (T) lymphocytes on the day of secondary antigen administration, implying that significant changes occur in the precursor population in the absence of T cells. This was supported by the finding that antigen-binding cells (ABC), the presumed precursors of antibody-secreting cells, proliferated normally in Tx-BM mice. However, instead of the replacement of µ-bearing ABC by significant numbers of ABC bearing other classes of immunoglobulin, as seen in the normal response to antigen, the ABC in Tx-BM animals possessed µ-receptors throughout the immune response. It is suggested that the generation or activation of non-µ-bearing ABC is strikingly impaired in the absence of a normal complement of T cells and may be responsible for the decreased IgG antibody production.

Footnotes

1 This work was supported by Grant AI-11635 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, a grant from the following companies: Brown & Williamson Tobacco Corporation; Larus and Brother Company, Inc.; Liggett & Myers Incorporated; Lorillard, a Division of Loews Theatres, Incorporated; Philip Morris, Incorporated; R. J. Reynolds Tobacco Company; United States Tobacco Company; and Tobacco Associates, Inc., and Grant IM-29 from the American Cancer Society, Missouri Division.




This article has been cited by other articles:


Home page
 Cold Spring Harb Symp Quant BiolHome page
M. D. Cooper, J. F. Kearney, P. M. Lydyard, C. E. Grossi, and A. R. Lawton
Studies of Generation of B-cell Diversity in Mouse, Man, and Chicken
Cold Spring Harb Symp Quant Biol, January 1, 1977; 41(0): 139 - 145.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1974 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1974 by The American Association of Immunologists, Inc. All rights reserved.