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Expression of New Antigens by HL-A Variants of Cultured Lymphoid Cells¹

Department of Pediatrics, University of Washington, School of Medicine, Seattle, Washington 98195

Abstract

Antisera were raised in rabbits to a number of spontaneously occurring and mutagen-induced HL-A2 loss variants of the cultured lymphoid line 8866. The antisera to the variant sublines were absorbed with 8866 and with one or both of the spontaneously occurring variant sublines, and the absorbed antisera were tested to determine whether they retained reactivity for the variant against which the antiserum was raised. A positive result was taken as evidence that the variant expressed a new antigen. By this criterion, neither spontaneously occurring variant appeared to express a new antigen. However, at least two of four mutagen-induced variants appeared to do so. Antiserum against 8866 absorbed with either of the spontaneously occurring HL-A2 loss variants appeared to be a specific HL-A2 reagent, supporting the idea that antigen loss in these variants is limited to the HL-A2 allele.

Footnotes

¹ This research supported by United States Public Health Service Grants GM15883 and HD05961.

² Recipient of a Research Career Development Award from the National Institute of Child Health and Human Development.

This article has been cited by other articles:

				D Pious and C Soderland HLA variants of cultured human lymphoid cells: evidence for mutational origin and estimation of mutation rate Science, August 19, 1977; 197(4305): 769 - 771. [Abstract] [PDF]