HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rouse, B. T. Articles by Babiuk, L. A. Search for Related Content PubMed Articles by Rouse, B. T. Articles by Babiuk, L. A.

Host Responses to Infectious Bovine Rhinotracheitis Virus

III. Isolation and Immunologic Activities of Bovine T Lymphocytes¹

Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada, S7N 0W0

Abstract

An investigation was made to determine if the nylon wool column technique of Julius et al., (9), could be used to fractionate bovine peripheral blood lymphocytes into T enriched (effluent) and B enriched (adherent) cells. After column filtration, the subpopulations were checked for surface-labeling characteristics with fluorescein-labeled anti-Ig serum (membrane fluorescence), phagocytic activity, response to mitogens, and their reactivity in two in vitro assays that quantitate cell-mediated immunity (CMI) against a bovine herpes virus-infectious bovine rhinotracheitis (IBR) virus. The effluent cells appeared enriched in T cells since few cells showed membrane fluorescence and these cells responded well to the T cell mitogens phytohemagglutinin (PHA) and concanavalin A (Con A). The adherent cells appeared enriched in B cells since many cells showed membrane fluorescence and the cells responded well to lipopolysaccharide (a B cell mitogen) but poorly to PHA and Con A. In the in vitro CMI assays against IBR virus, the effluent cells were far more reactive than the adherent cells implying that these assays are essentially measures of T cell activity.

The implications of the results for the understanding of the mechanism of resistance to herpes virus infections is discussed.

Footnotes

¹ This work was supported by Grant MA5067 of the Medical Research Council of Canada.

This article has been cited by other articles:

				X. P. Ioannou, P. Griebel, R. Hecker, L. A. Babiuk, and S. van Drunen Littel-van den Hurk The Immunogenicity and Protective Efficacy of Bovine Herpesvirus 1 Glycoprotein D plus Emulsigen Are Increased by Formulation with CpG Oligodeoxynucleotides J. Virol., August 12, 2002; 76(18): 9002 - 9010. [Abstract] [Full Text] [PDF]

				V. Gerdts, M. Snider, R. Brownlie, L. A. Babiuk, and P. J. Griebel Oral DNA Vaccination In Utero Induces Mucosal Immunity and Immune Memory in the Neonate J. Immunol., February 15, 2002; 168(4): 1877 - 1885. [Abstract] [Full Text] [PDF]