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Studies on the Induction and Expression of T Cell-Mediated Immunity. III. Amplification by Papain of the Generation of Cytotoxic Lymphocytes to Allogeneic Cells¹

From the Department of Microbiology and Immunology, UCLA School of Medicine, Los Angeles, California 90024

Abstract

We have demonstrated in a recent study (1) that mild proteolytic treatment of immune cytotoxic lymphocytes (CL)² leads to a 3- to 5-fold increase in the in vitro cytolytic activity upon target cells bearing the sensitizing antigens. In the present report we describe the potentiation of the generation of CL after treatment of normal lymphoid cells with low concentrations of papain. Thus, BALB/c (H-2^d) splenocytes treated with papain and subsequently sensitized in vitro or in vivo against allogeneic C57BL/6 (H-2^b) lymphoid cells exhibit an enhanced specific cytotoxic capacity as compared with untreated cells. Furthermore, the induction of CL with papain-treated cells occurs under conditions in which non-treated cells show little or no reactivity.

Materials and Methods. The preparation of spleen cells and enzymatic treatment with papain were described previously (1).

Footnotes

¹ This work was supported by Grant CA 12800 from the National Cancer Institute, National Institutes of Health, Bethesda, Maryland.

² Abbreviations used in this paper; CL, cytotoxic lymphocytes; GVH, graft-vs-host; MLC, mixed lymphocyte culture; CMC, cell-mediated cytotoxicity.