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Development of Humoral Immunity in Lethally Irradiated Mice Reconstituted with Fetal Liver¹

From the Department of Pathology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19174

Abstract

A model system of ontogeny was utilized to investigate the development of humoral immunity. Lethally irradiated adult C3H mice were reconstituted with syngeneic fetal liver. These mice were immunized at various times after reconstitution with a series of eight antigens: the bacteriophages F2 T4 and øX-174; the hapten carrier complexes 2,4 dinitrophenyl-bovine serum albumin and fluorescein bovine serum albumin; and the small proteins, hen egg lysozyme, sperm whale myoglobin, and bovine pancreatic ribonuclease. Subsequent antibody production to the bacteriophage was assayed using a bacteriophage neutralization technique. To detect antibody directed against the haptens or small proteins, the appropriate hapten or protein was first coupled to T4, and then a modified bacteriophage neutralization technique was employed. Individual mice responded to the various antigens in a sequential pattern which was basically the same for all mice within the strain. This hierarchy of responsiveness could not be explained on the basis of different immunogenicities of the antigens nor on different sensitivities of the antibody assays. Instead, we propose that the hierarchy results from the ordered maturation of lymphopoietic elements in the fetal liver inoculum.

Footnotes

¹ This work was supported in part by National Institutes of Health Grant 02046 (M.S.T.P.).

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