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Human Lymphotoxin Obtained from Established Lymphoid Lines: Purification Characteristics and Inhibition by Anti-Immunoglobulin¹

From the Thyroid Study Unit, Department of Medicine, University of Chicago, Chicago, Illinois 60637, and Department of Surgery, Denver General Hospital, Denver, Colorado 80204

Abstract

Human lymphotoxin obtained from established lymphoid cell lines (HLT-LCL) was studied in detail and purified using ammonium sulfate fractionation, gel filtration, ion-exchange column chromatography, and polyacrylamide gel electrophoresis. HLT-LCL is stable at 20°C for over 1 year, at 4°C for 3 weeks, and at 37°C for 1 week. HLT-LCL showed a decrease in activity at pH 2.0 and 3.0, but was stable at a pH range of 5.0–11.0 HLT-LCL, which contained fetal calf serum, was purified 50-fold. HLT-LCL obtained from serum-free culture medium yielded greater purity, although complete purification was not achieved. Antiserum to HLT-LCL neutralized the cytotoxic activity of HLT-LCL, HLT induced by PHA (HLT-PHA), and HLT induced by PPD (HLT-PPD). Antiserum to human IgG, -type L chain, and Fc fragment partially but significantly inhibited HLT-LCL activity. These data, together with previous reports, indicate that the HLT-LCL molecule is immunologically similar to HLT-PHA and HLT-PPD and suggest that there is immunologic cross-reactivity with IgG globulin.

Footnotes

¹ Supported by American Cancer Society Grant 71-34 and United States public Health Service Grant AM-13,377.