The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 1974, 113: 1223-1229.
Copyright © 1974 by The American Association of Immunologists, Inc.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gerety, R. J.
Right arrow Articles by Barker, L. F.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Gerety, R. J.
Right arrow Articles by Barker, L. F.

Humoral and Cell-Mediated Immune Responses to Two Hepatitis B Virus Antigens in Guinea Pigs1

Robert J. Gerety2, Jay H. Hoofnagle and Lewellys F. Barker

From the Bureau of Biologics, Food and Drug Administration, 8800 Rockville Pike, Bethesda, Maryland 20014

Abstract

The ability of two purified, particulate hepatitis B virus antigens to induce antibodies and delayed cutaneous hypersensitivity responses was studied in Hartley guinea pigs. Hepatitis B surface antigen (HBsAg) was purified by several methods from the plasmas of human, chronic HBsAg carriers, while hepatitis B core antigen (HBcAg) was purified from chimpanzee liver by ultracentrifugation. Guinea pigs immunized with the HBsAg preparations developed anti-HBs in the absence of anti-HBc but delayed cutaneous hypersensitivity reactions to HBsAg were seen clearly only in animals immunized with high doses of the most highly purified HBsAg preparation. Guinea pigs immunized with the HBcAg preparation developed anti-HBc in the absence of anti-HBs, and showed delayed cutaneous hypersensitivity reactions to HBcAg in the absence of reactions to a chimpanzee liver-derived control antigen. No skin reactivity was seen to HBcAg in guinea pigs immunized with HBsAg. Similarly, guinea pigs immunized with HBcAg showed no skin reactivity to HBsAg. It was concluded that both HBsAg and HBcAg are capable of inducing distinct humoral and cell-mediated immune responses in Hartley guinea pigs.

Footnotes

1 Presented in part at the Federation of American Societies for Experimental Biology and Medicine, Atlantic City, New Jersey, May 1974.

2 Address reprints to: Robert J. Gerety, Hepatitis Branch, Bureau of Biologics, Food and Drug Administration, 8800 Rockville Pike, Bethesda, Maryland 20014.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1974 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1974 by The American Association of Immunologists, Inc. All rights reserved.