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Studies on the Human T-Lymphocyte Population

II. The Use of a T-Cell Specific Antibody in the Partial Isolation and Characterization of the Human Lymphocyte Receptor for Sheep Red Blood Cells¹

From the Department of Microbiology, Case Western Reserve University, Cleveland, Ohio 44106

Abstract

An IgG fraction of a goat antibody prepared against human thymus cells and absorbed with red blood cells, insolubilized human Ig, and cells from a patient with chronic lymphocytic leukemia (ATG) has been found to react specifically with the human T lymphocyte population (1). This antibody blocked spontaneous rosette formation by human peripheral lymphocytes and sheep red blood cells (SRBC). The F(ab')₂ and Fab' fragments of ATG were also effective in blocking rosette formation. Moreover, capping of SRBC on lymphocytes was observed when cells were incubated with the F(ab')₂ fragment of ATG, but not with the Fab' fragment. The data suggest that on human peripheral lymphocytes the receptor for SRBC and a T-specific antigen may be the same or may be linked. A soluble extract of human peripheral lymphocytes contained material capable of reacting with ATG. Fractions of this extract obtained from Sephadex G-200 and G-75 columns and corresponding to molecular weights of approximately 35,000 and 70,000 were capable of blocking ATD's inhibition of rosette formation. The active components appeared to be glycoproteins and migrated with Rf values of 0.96 on acrylamide gel electrophoresis.

Footnotes

¹ This work was supported by Research Grants AI10148 from the United States Public Health Service GM00171.

² United States Public Health Service Career Development Awardee, AI25732.