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In Vitro Studies on Allotype Suppression

I. Release from Allotype Suppression by Antibodies Specific for the Non-Suppressed Allotype¹

From the Department of Immunology, The Public Health Research Institute of The City of New York, Inc., New York, New York 10016

Abstract

Spleen cells from heterozygous rabbits with b locus immunoglobulin allotypes b4 and b5 which had been exposed in utero to maternal antibodies against the paternal allotype (b4) were stimulated in vitro with solubilized T2 phage antigen. The cells of thoroughly suppressed rabbits formed T2 neutralizing antibodies which were identifiable only as having allotype b5. The presence of anti-b5 serum in the cultures inhibited b5 expression and resulted in the formation of antibodies having the previously suppressed allotype, b4. Normal rabbit serum of the same allotypic specificity as the anti-b5 serum (b4) had no effect on the allotype distribution of anti-T2 antibodies. Although partial inhibition of b5 production occurred when the anti-b5 serum was removed from cultures after 5 hr, no reversal of b4 suppression was observed. Possible mechanisms accounting for these results are discussed.

Footnotes

¹ This work was supported in part by Grant GB-29018X from the National Science Foundation.