The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 1974, 113: 885-895.
Copyright © 1974 by The American Association of Immunologists, Inc.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Leung, C. C. K.
Right arrow Articles by Brent, R. L.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Leung, C. C. K.
Right arrow Articles by Brent, R. L.

Evidence That Different Antibodies Are Involved in the Production of Immunologically Induced Teratogenesis and Nephritis1

Christopher C. K. Leung, Antonio Urdaneta2, Ronald P. Jensh, Marcela Jensen and Robert L. Brent

From the Stein Research Center and the Department of Pediatrics, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

Abstract

Since it has been well established that heterologous antiserum against whole rat kidney is both teratogenic and nephrotoxic, it is important to determine if these two separate pathologic effects are caused by the same or a different group of antibodies. Two antisera were prepared from the following groups of soluble antigens: (1) GBM antigens were obtained by solubilizing isolated rat glomerular basement membrane with trypsin, (2) PBS-soluble kidney antigens were obtained from the supernatant of homogenized, perfused whole rat kidneys following centrifugation at 105,000 x G. Both antisera were assayed for teratogenicity and nephrotoxicity. Nephrotoxicity of the antisera was evaluated by measuring proteinuria and examining light and electron microscopic preparations of kidneys following the injection of antisera into male rats and pregnant rats. The antiserum to trypsin-solubilized GBM antigens was nephrotoxic but not teratogenic while the antiserum to PBS-soluble kidney antigens was teratogenic but not nephrotoxic. In addition, the results of absorption studies utilizing antiserum against whole rat kidney indicate that a) the teratogenic effect of the antiserum against whole rat kidney can be abolished by incubating the antiserum with PBS-soluble kidney antigens, b) the nephrotoxic effects of the antiserum against whole rat kidney still persists after absorbing the antiserum with PBS-soluble kidney antigens. In vivo immunofluorescent studies demonstrated that the antibodies to PBS-soluble kidney antigens localized in the maternal GBM, Reichert's membrane, and visceral yolk sac endodermal cells whereas the antibodies to trypsin-solubilized GBM antigens localized only in the maternal GBM. These data indicate that the teratogenic antibodies present in the classical (Masugi) nephrotoxic serum are not the same group of antibodies that are responsible for nephrotoxicity.

Footnotes

1 This work was presented in part at the 57th Annual Meeting of the American Association of Immunologists, Atlantic City, New Jersey, April, 1973, and supported by National Institutes of Health Grants HD 630 and HD 370.

2 Research Postdoctoral Fellow, Universidad de Los Andes, Department de Farmacologia, Merida, Venezuela.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1974 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1974 by The American Association of Immunologists, Inc. All rights reserved.