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Isolation of Recombinant IgG Molecules Carrying Heavy Chain Allotypes Transmitted in Repulsion

From the Laboratory of Cell Biology, C.N.R., Via Romagnosi 18A, Roma, Italy, and Istituto Regina Elena per lo Studio e Cura dei Tumori, Roma, Italy

Abstract

Recombinant IgG molecules possessing the heavy chain markers transmitted in repulsion were isolated from a rabbit serum and analyzed. The rabbit genotype was a1/a2; e14/e15, a1 being in coupling with e14 and a2 with e15. Recombinant molecules carrying either a1 and e15 or a2 and e14 were obtained in a relatively pure form by sequential absorption and elution with cross-linked antisera. a1–e15 molecules also were compared by immunoelectrophoresis with other IgG fractions, "nonrecombinant" IgG and "a-minus" IgG, isolated from the same serum. All fractions showed similar immunoelectrophoretic patterns. a1–e15 recombinant molecules were found to have a normal : light chain ratio and a normal -chain allotypes ratio. Furthermore, they could be dissociated into heavy and light chains which elute after gel filtration at the same positions as nonrecombinant molecules.

Footnotes

¹ Laboratory of Cell Biology, C.N.R., Via Romagnosi 18A, Roma, Italy.

² Instituto Regina Elena per lo Studio e Cura dei Tumori, Roma, Italy.