HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Anaclerio, A. Articles by Möller, G. Search for Related Content PubMed Articles by Anaclerio, A. Articles by Möller, G.

Induction of Lymphocyte-Mediated Cytotoxicity against Allogeneic Tumor Cells by Concanavalin a in Vivo¹

From the division of Immunobiology, Karolinska Institutet, Wallenberglaboratory, Lilla Freskati, 104 05 Stockholm 50, Sweden

Abstract

The in vivo administration of concanavalin A (Con A) induced a dose and time dependent cytotoxic response in spleen cell populations when tested against allogeneic target cells in vitro. Con A doses between 60 µg and 480 µg induced cytotoxicity, which was maximal 24 hr after injection and thereafter disappeared. Cytotoxicity correlated with the time of maximum DNA synthesis and blast transformation in the spleen. The addition of extra Con A to the in vitro test system did not change the kinetics or intensity of cytotoxicity. The results are discussed in terms of the effect of Con A in the activation of cytotoxic lymphocytes and its possible further role in effecting "killer-target" cell binding.

Footnotes

¹ This work was supported by grants from the Swedish Cancer Society, the Swedish Medical Research Council, the Wallenberg Foundation and the Jeansson Foundation. Dr. A. Anaclerio was supported by a fellowship from the Swedish Institute and by the Italian National Research Council.

² Dr. Alessandro Anaclerio, Department of Medicine, Roger Williams General Hospital and Brown University, Division of Biology and Medical Sciences, Providence, Rhode Island.