HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Marrack, P. Articles by Kettman, J. R. Search for Related Content PubMed Articles by Marrack, P. Articles by Kettman, J. R.

The Frequency and Activity of Single Helper T Cells¹

From the Department of Microbiology, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, and the Department of Biology, University of California at San Diego, La Jolla, California 92037

Abstract

We have investigated the activities of T cell populations able to cooperate in the humoral immune response to TNP coupled to the carriers SRBC or KLH. We used Poisson analyses in which small numbers of carrier-primed spleen cells were added to cultures containing an excess of B cells and antigen. We have demonstrated that we could a) detect the activity of a single helper T cell; b) calculate its frequency in a given spleen cell suspension; and c) calculate its relative activity in stimulating the humoral immune response.

Footnotes

¹ This work was supported by United States Public Health Service Research Grant CA-11190, A-11558 and A-08795 and American Cancer Society Research Grant 1C-1E.

² Present address: Department of Microbiology, Southwestern Medical School at Dallas, 5323 Harry Hines, Dallas, Texas 75235.

This article has been cited by other articles:

				W. Rees, J. Bender, T. K. Teague, R. M. Kedl, F. Crawford, P. Marrack, and J. Kappler An inverse relationship between T cell receptor affinity and antigen dose during CD4+ T cell responses in vivo and in vitro PNAS, August 17, 1999; 96(17): 9781 - 9786. [Abstract] [Full Text] [PDF]

				S. L. Swain, P. E. Trefts, H. Y.-S. Tse, and R. W. Dutton The Significance of T-B Collaboration across Haplotype Barriers Cold Spring Harb Symp Quant Biol, January 1, 1977; 41(0): 597 - 609. [Abstract] [PDF]