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Anti-Histone KSA_chicken Antibody from Guinea Pig¹

From the Department of Biochemical and Biophysical Sciences, The Johns Hopkins University, School of Hygiene and Public Health, Baltimore, Maryland 21205

Abstract

Avian erythrocyte-specific histone KSA was purified from chicken red blood cells by selective extraction and by chromatography on amberlite CG-50 columns. This histone was used in the production of antibody in guinea pigs. By immunoprecipitation and microcomplement-fixation tests, the antibody was shown to be specific for histone KSA; reaction was not seen with histones KAP, ARE, GRK, LAK, and KAS. The observation supports the notion that histone KSA has an independent origin from the other histones.

Footnotes

¹ This work was supported by grants from the National Science Foundation, the National Foundation/March of Dimes, and the National Institutes of Health.

² Casilda Mura is on leave from the Universidad de Chile and a holder of AAUW International Postdoctoral Fellowship.

³ Please address correspondence to P. C. Huang.