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Importance of Age of Host in Detecting Tumor-Associated Transplantation Antigens¹

From the Wistar Institute of Anatomy and Biology, 36th Street at Spruce, Philadelphia, Pennsylvania 19104

Abstract

We report here the importance of the age of the host in the detection of immunogenic tumor-specific transplantation antigens (TSTA)³ in transplantation experiments in vivo. Demonstration of the presence of TSTA on the K-BALB cell line, a BALB/3T3 cell line transformed by the Kirsten strain of murine sarcoma virus (MSV) but not producing virus, was dependent on the age of the BALB/c mice immunized.

Stephenson and Aaronson (1) previously failed to detect tumor-associated transplantation antigens (TATA) on this same cell line when they performed transplantation immunity studies in 6- to 8-week-old BALB/c mice. Their negative conclusion was based, in part, upon observing comparable protection when using K-BALB and the parental BALB cell lines as vaccines. The authors admitted that the BALB/3T3 parental cell line may have undergone antigenic changes concomitant with prolonged passage in vitro Furthermore, we (unpublished observation) and others (2) have found that the putative normal BALB/3T3 cell line is, in fact, weakly tumorigenic.

Footnotes

¹ This work was supported in part by Contracts 71-2092 and CP 33250 within the Virus Cancer Program of the National Cancer Institute and United States Public Health Service.

³ Abbreviations used in this paper: TSTA, tumor-specific transplantation antigen; MSV, murine sarcoma virus; TATA, tumor-associated transplantation antigen; MEM, Eagle's minimal essential medium; FCS, fetal calf serum; i.p., intraperitoneally; s.c., subcutaneously.

² Present address: Winter Research Laboratory, Mt. Sinai Hospital, 948 N. 12th Street, Milwaukee, Wisconsin 53233.