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Effect of Carrier Priming on the Distribution of Anti-Hapten Antibodies between IgG1 and IgG2 Isotypes in the Hyperimmune Guinea Pig¹

Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218

Abstract

Priming of guinea pigs with a soluble carrier protein, keyhole limpet hemocyanin, can have a marked influence on the isotype distribution between IgG1 and IgG2 of anti-dinitrophenyl or anti-p-azobenzenearsonate antibodies produced upon subsequent immunization with hapten-carrier conjugates. Lowdose carrier priming (40 µg) causes a marked shift from IgG2 to IgG1 anti-hapten antibody without affecting the total amount of antibody produced compared with the anti-hapten response of animals directly immunized with conjugates. High-dose carrier priming (300 µg) enhances the overall anti-hapten response by causing an increase in the amount of IgG2 anti-hapten antibody produced while an equivalent amount of IgG1 antibody to that made after low dose carrier priming is also synthesized. Preliminary studies indicate that injection of a low dose of soluble carrier depresses MIF release by carrier-reactive T lymphocytes. We postulate that such prior injection of carrier also affects the balance between the "helper function" reactivity of carrier-specific T cells and the sensitivity to stimulation of different isotope-committed B cells.

Footnotes

¹ This work was supported by NSF Grant GB25471 and NIH Grant AI-09652.

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