HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Litwin, S. D. Articles by Cleve, H. Search for Related Content PubMed Articles by Litwin, S. D. Articles by Cleve, H.

Immunoglobulin Expression of Cells from Human Lymphoblastoid Lines

I. Heavy and Light Chain Antigens of the Cell Surface^1,2,

Division of Human Genetics, Department of Medicine, Cornell University Medical College, New York, New York 10021

Abstract

Surface Igs of eight different human established lymphoblastoid lines were investigated by means of a radioassay method and cytotoxicity testing. The number of surface Ig sites varied from 1.0 to 4.5 x 10⁵ per Ig positive cell in different lymphoid lines. µ heavy (H) chain and light (L) chains were the predominant surface Ig antigens encountered in most but not all cases. A molar excess of L chains was observed in five out of eight lymphoid lines. Multiple factors contributed to the excess of L chains. In certain lines a L chain excess was also present in secreted and in cellular Ig favoring an interpretation of unbalanced oversynthesis of L chains. Two lines, however, had the L chain excess restricted to surface Ig. Experiments on two of the lines with L chain excess demonstrated that while most Ig positive cells had both L and H chain surface antigens, a minor proportion of cells had only L chains detectable on their surface.

Footnotes

¹ This work was supported in part by United States Public Health Service Grants AI 09239, AM 20122, and AM 11796. P. K. Lin is a post-doctoral trainee (United States Public Health Service Training Grant GM 01918).

² This work was presented in part at the National Meeting of the Federation of American Societies for Experimental Biology, Atlantic City, New Jersey, April 1973.

This article has been cited by other articles:

				E. Vitetta and J. Uhr Immunoglobulin-receptors revisited Science, September 19, 1975; 189(4207): 964 - 969. [PDF]