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Immunologic Reactions to Haptens on Autologous Carriers

III. Cell Selection at the Antigen-Specific in Vitro DNA Synthetic Response Level¹

Division of Clinical Immunology, Departments of Medicine and Microbiology, University of Colorado Medical Center, Denver, Colorado 80220

Abstract

Antigen-induced lymphocyte proliferation in vitro was used to investigate changes in receptor affinity of cells from mice immunized with haptens coupled to autologous carriers. We attempted: 1) to show a differential sensitivity to antigen in vitro after priming with low or high doses of antigen in vivo; 2) to assess the in vitro response to different doses of antigen at various times after priming with a single dose of antigen; 3) to demonstrate high affinity cells by blocking the in vitro response with low doses of free hapten. Using all three procedures, we were unable to demonstrate either a selection of high affinity cells or a maturation of cells with high affinity receptors with time after immunization. The results suggest that in mice, hapten coupled to autologous carriers are weak immunogens and are unable to prime a majority of cells with high affinity receptors.

Footnotes

¹ This work was supported by National Institutes of Health Grant AM-10145.

² Special Fellow of the Leukemia Society.