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Isolation of Mouse Thymus-Derived Lymphocyte Specific Surface Antigens¹

Universite de Lausanne, Institut de Biochimie, 21 rue du Bugnon, CH-1005 Lausanne, Switzerland

Abstract

Mouse T lymphocyte-specific xenoantigens (MTLA) and alloantigen O have been isolated by Sephadex chromatography and preparative polyacrylamide gel electrophoresis of SDS-stabilized mouse thymocyte membranes. The MTLA xenoantigen and the alloantigen activities, as tested by micro-complement fixation and inhibition of lymphocytotoxicity are detected on a protein of an apparent molecular weight between 35 and 40,000 daltons. A 120-fold enrichment of the xenoantigen and 60-fold of the alloantigen has been obtained. Immunization of AKR mice with purified material produced anti- alloantiserum. By immunization of rabbits with 50 µg to 100 µg of purified protein, potent antisera have been obtained which are specific for mouse T cells and do not require any absorption procedure.

Footnotes

¹ This work was supported by a Swiss National Scientific Foundation Grant 3.061.73.