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In Vitro Antibody Formation by Human Tonsil Lymphocytes¹

Third Department of Internal Medicine, Osaka University Medical School, Fukushima-ku, Osaka, 553, Japan

Abstract

In vitro antibody formation by human lymphocytes was observed against hapten-protein conjugates by using human palatine tonsil cells in the presence of an optimum concentration of 2-mercaptoethanol. Relatively pure populations of B and T lymphocytes were prepared by the method of Ficoll-Hypaque gradient centrifugation to investigate cellular mechanism of antibody formation by human lymphocytes. Maximum response to hapten-protein conjugates was observed in vitro when T and B cells were mixed at an optimal ratio. Little response occurred when each cell alone was cultured with antigen. Depletion of glass-adherent cells also resulted in diminution of the anti-hapten response. These data indicated that three kinds of cells—T cells, B cells, and glass-adherent cells—are essentially necessary for in vitro primary antibody formation by human tonsil cells.

Footnotes

¹ A part of this study was presented at the meeting of 8th International Congress of Allergology at Tokyo in October 1973.

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