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The Use of Protein-Substituted Nylon Catheters for Selective Immunoadsorption in Vivo¹

Washington University School of Medicine, Department of Medicine, St. Louis, Missouri 63110

Abstract

Nylon tubing was partially hydrolyzed with acid and conjugated with human serum albumin (HSA) or ovalbumin (OA) by means of a water soluble carbodiimide or glutaraldehyde. The antigen-substituted tubing reacted specifically with antibody in vitro as indicated by selective uptake of ¹²⁵I-labeled anti-HSA or ¹³¹I-labeled anti-OA from mixtures of the two radioactive proteins. The HSA-substituted tubing had a ¹²⁵I to ¹³¹I ratio of ; 2.5 whereas the OA-substituted tubing had a ratio of < 0.4. The antigen-substituted tubing was evaluated for its ability to interact with antigen in vivo by passing lengths of HSA and OA tubing into the inferior vena cava of heparinized dogs. As in the in vitro experiments selective binding of ¹²⁵I anti-HSA or ¹³¹I anti-OH antibody was observed. Kinetic measurements indicated that if the tubing were cycled continuously through the venous system it would be possible theoretically to remove milligram amounts of antibody over a 24-hr period. Since nylon tubing can be conjugated to a variety of proteins, haptens, and hapten protein conjugates, the nylon catheter system may be applicable to the removal of circulating antigens and antibodies in man and experimental animals.

Footnotes

¹ This work was supported by United States Public Health Service Training Grant AI00219.