HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vischer, T. L. Search for Related Content PubMed Articles by Vischer, T. L.

Stimulation of Mouse B Lymphocytes by Trypsin^1,2,

From the Division of Rheumatology, Department of Medicine, University of Geneva, Hôpital Beau-Séjour, 1211-Geneva, Switzerland

Abstract

BALB/c spleen cells (5 x 10⁶) were cultured in 1 ml of serum-free RPMI 1640 medium for 3 days. Stimulation was assessed after addition of ³H-thymidine for the last 16 hr. Addition of trypsin to the cultures resulted in a stimulation comparable to that induced by endotoxin (LPS). Optimal amounts for stimulation were around 2.5 µg. Trypsin had to be active during at least 24 hr of cultures for stimulation as determined by addition of soybean trypsin inhibitor. Thymus cells from normal or hydrocortisone-treated mice could not be stimulated whereas spleen and lymph node cells from homozygous, athymic, nude mice responded well. Similarly, over 80% of the transformed cells after stimulation of BALB/c spleen cells by trypsin had immunoglobulin surface receptors and are B cells. Removal of adherent cells from the cultures did not diminish the response to trypsin or LPS. It is concluded that trypsin is a potent stimulator of B lymphocytes.

Footnotes

¹ This work was presented in a preliminary form at the Joint Meeting of the European Societies for Immunology, Strasbourg, France, September 1973.

² This work was supported by the Swiss National Fund for Scientific Research.

This article has been cited by other articles:

				E. Vitetta and J. Uhr Immunoglobulin-receptors revisited Science, September 19, 1975; 189(4207): 964 - 969. [PDF]

				P. M. C. Wong, Q. Yuan, H. Chen, B. M. Sultzer, and S.-W. Chung A Single Point Mutation at the 3'-Untranslated Region of Ran mRNA Leads to Profound Changes in Lipopolysaccharide Endotoxin-mediated Responses J. Biol. Chem., August 24, 2001; 276(35): 33129 - 33138. [Abstract] [Full Text] [PDF]