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Interaction of Isoantibody and Cytotoxic Lymphocytes with Allogeneic Tumor Cells¹

From the Memorial Sloan-Kettering Cancer Center, Walker Laboratory, Rye, New York 10580

Abstract

Specific isoantibody against DBA/2J mastocytoma cells was purified from immune serum by immunoadsorption without losing its inhibiting activity of lymphocyte-mediated cytotoxicity (LMC). The molecular nature of the isoantibody was studied with ¹²⁵I-labeled antibody (¹²⁵I-IS). The Ig nature was verified by specific serology with rabbit anti-mouse Ig, acrylamide gel analysis, and specific binding to mastocytoma cells.

The fate of ¹²⁵I-IS on the surface of activity metabolizing mastocytoma cells was studied. Almost all ¹²⁵I-IS are bound to mastocytoma cells, inhibiting T cell-mediated cytotoxicity. At the time of the onset of the "escape" from IS inhibition of LMC, 20 to 30% of bound ¹²⁵I-IS was released and the surface antigens were regenerated. This phenomenon is also temperature dependent, requiring active metabolism of the target cells.

Footnotes

¹ This work was supported by Grants CA08748 and AI-10,415 from the U.S. Public Health Service, IC-50 from the American Cancer Society, the Damon Runyon Memorial Fund and the Jane Coffin Childs Memorial Fund.