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Tissue Distribution of I Region-Associated Antigens in the Mouse¹

Vra Hauptfeld², Miroslav Hauptfeld² and Jan Klein²

From the Department of Human Genetics, The University of Michigan Medical School and the Department of Oral Biology, The University of Michigan School of Dentistry, Ann Arbor, Michigan 48104

Abstract

Antiserum (A.TL x B10)F₁ anti-B10.HTT, obtained after skin grafting and immunization with spleen and lymph node cells, contains antibodies against antigens controlled by the I region of the H-2 complex (Ia antigens). Two additional Ia antigens have been identified, antigen Ia.3 which is restricted to the H-2^s haplotype and its recombinant derivatives H-2^t2 and H-2^t3, all showing the same Ir-1A^s subregion, and antigen Ia.4 which is shared by H-2 haplotypes f, r, and u. The anti-Ia antibodies react in a direct cytotoxic test with about 40 to 50% lymph node cells and 50 to 60% spleen cells, but do not react with thymus or bone marrow cells. However, the anti-Ia antibodies can be absorbed in vitro with large numbers of cells by both thymus and bone marrow cells; they cannot be absorbed with muscle, erythrocytes, and brain tissue. After specific removal of anti-Thy-1.1-sensitive cells from the spleen cell suspension, the proportion of cells killed by the anti-Ia sera increases to almost 90%. Pretreatment in vivo with cyclophosphamide abolishes the anti-Ia reactivity of spleen cells. Suspensions filtered through a nylon fiber column are enriched in Ia-negative cells, the Ia-positive cells being retained on the fibers. Pretreatment in vivo with hydrocortisone results in the appearance of a small fraction of Ia-positive cells in the thymus. Thus, the Ia antigens are probably restricted to lymphocytes with predominant expression on B cells; if T cells express these antigens at all they do so either with a very low surface density or only in a minor subpopulation.

Footnotes

¹ This work was supported by Public Health Service research Grants GM15419 and DE02731.

² Present address: Department of Microbiology, The University of Texas Southwestern Medical School, Dallas, Texas 75235.